| FAM19A5

Chemokine-like protein TAFA-5 is a protein that is encoded by the TAFA5 gene.This gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted proteins. These proteins contain conserved cysteine residues at fixed positions, and are distantly related to CCL3, a member of the CC-chemokine family. The TAFA proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines, that act as regulators of immune and nervous cells.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	5000	2500	1250	625	312	156	78	0
OD	2.36	1.34	0.8	0.48	0.34	0.26	0.22	0.09
Corrected OD	2.27	1.25	0.71	0.39	0.25	0.17	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human FAM19A5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	83-101	96
Cell Culture Supernates(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human FAM19A5 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	86-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	95	98